Genetic Relationships Within and Between Capsicum Species

İNCE A. G., KARACA M., ONUS A. N.

BIOCHEMICAL GENETICS, cilt.48, sa.1-2, ss.83-95, 2010 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 48 Sayı: 1-2
  • Basım Tarihi: 2010
  • Doi Numarası: 10.1007/s10528-009-9297-4
  • Dergi Adı: BIOCHEMICAL GENETICS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.83-95
  • Anahtar Kelimeler: RAPD-PCR, DNA markers, Species relationships, Capsicum complex, Touch-down PCR, FRAGMENT-LENGTH-POLYMORPHISM, GENUS CAPSICUM, RAPD MARKERS, CYNODON SPP., SOLANACEAE, DIVERSITY, CHLOROPLAST, ACCESSIONS, WILD, REGIONS
  • Akdeniz Üniversitesi Adresli: Evet

Özet

Genetic relationships were estimated among 24 accessions belonging to 11 species of Capsicum, using 2,760 RAPD markers based on touch-down polymerase chain reactions (Td-RAPD-PCR). These markers were implemented in analyses of principal coordinates, unweighted pair group mean average, and 2,000 bootstrap replications. The accessions were divided into four groups, corresponding to previously described Capsicum complexes: C. annuum complex (C-A), C. baccatum complex (C-B), C. pubescens complex (C-P), and C. chacoense accessions (C-A/(B)). Their overall mean genetic similarity index was 0.487 +/- A 0.082, ranging from 0.88 to 0.32, based on Jaccard's coefficient. The highest genetic variation was observed among the accessions in C-P; the accessions in C-B had a low level of variation as judged from the standard deviations of the genetic similarity indices. Based on the Td-RAPD-PCR markers, the 24 accessions were divided into four major groups, three of which corresponded to the three distinct Capsicum complexes. Accessions of C. chacoense were found to be equally related to complexes C-A, C-B, and C-P.