Matrix-Assisted Laser Desorption-Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) for Early Identification of Septic Patients

SEKERCIOGLU, Ali; Çekin, Yesim; OGUNC, MERAL; ÖNGÜT, GÖZDE; Baysan, BETİL; ÇOLAK, DİLEK; Gunseren, FİLİZ; DÖNMEZ, LEVENT

doi:10.7754/clin.lab.2016.160907

Matrix-Assisted Laser Desorption-Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) for Early Identification of Septic Patients

SEKERCIOGLU A. O., Çekin Y., OGUNC D., ÖNGÜT G., Baysan B., ÇOLAK D., ...Daha Fazla

CLINICAL LABORATORY, cilt.63, sa.4, ss.839-844, 2017 (SCI-Expanded, Scopus)

Yayın Türü: Makale / Tam Makale
Cilt numarası: 63 Sayı: 4
Basım Tarihi: 2017
Doi Numarası: 10.7754/clin.lab.2016.160907
Dergi Adı: CLINICAL LABORATORY
Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
Sayfa Sayıları: ss.839-844
Anahtar Kelimeler: MALDI-TOF MS, identification after short-term culture, bacteremia, candidemia, POSITIVE BLOOD CULTURES, SHORT-TERM INCUBATION, RAPID IDENTIFICATION, BACTERIA
Akdeniz Üniversitesi Adresli: Evet

Özet

Background: Matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a powerful technique for the rapid identification of bacteria from growing colonies in routine cultures. In this study, we evaluated the feasibility of a 5-hour incubation on solid medium after sub-cultivation of positive blood culture broth without any preparation steps in order to speed up the identification of bacteria.