A rapid molecular detection method for Aphelenchoides besseyi from rice tissues

DEVRAN Z., TULEK A., MISTANOGLU I., CIFTCIGIL T. H., OZALP T.

AUSTRALASIAN PLANT PATHOLOGY, cilt.46, sa.1, ss.43-48, 2017 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 46 Sayı: 1
  • Basım Tarihi: 2017
  • Doi Numarası: 10.1007/s13313-016-0452-1
  • Dergi Adı: AUSTRALASIAN PLANT PATHOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.43-48
  • Anahtar Kelimeler: Detection, PCR, rDNA, Ricewhite tip nematode, WHITE TIP NEMATODE, IDENTIFICATION, REGION, TURKEY, AREAS, SPP.
  • Akdeniz Üniversitesi Adresli: Evet

Özet

Rice is an important agricultural crop providing nutrition for humans worldwide. Rice white tip nematode, Aphelenchoides besseyi, is a nematode causing economic yield losses globally in rice-growing areas. Because A. besseyi is a seed-transmitted nematode, its rapid and accurate identification is important for seed certification and quarantine purposes. Here, we developed species-specific primers for the small subunit ribosomal DNA region of the Aphelenchoides genus to identify A. besseyi. DNA was extracted from juveniles and directly infected tissues, such as hulls, brown rice and paddy rice. A. besseyi was easily and rapidly detected from plant tissues without nematode extraction. Because the extraction of A. besseyi from plant tissues is time-consuming and labour-intensive, the developed method may be applied to seed certification and quarantine approaches. To our knowledge, this is the first report directly identifying A. besseyi from infected plant tissues.