Tracing of islet graft survival by way of in vivo fluorescence imaging

Kahraman S., Dirice E., Hapil F. Z., ERTOSUN M. G., ÖZTÜRK S., Griffith T. S., ...Daha Fazla

DIABETES-METABOLISM RESEARCH AND REVIEWS, cilt.27, sa.6, ss.575-583, 2011 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 27 Sayı: 6
  • Basım Tarihi: 2011
  • Doi Numarası: 10.1002/dmrr.1216
  • Dergi Adı: DIABETES-METABOLISM RESEARCH AND REVIEWS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.575-583
  • Anahtar Kelimeler: EGFP, in vivo imaging, adenovirus, type 1 diabetes, xenogeneic islet transplantation, TYPE-1 DIABETES-MELLITUS, LUNG-CANCER CELLS, ADENOVIRUS DELIVERY, TRANSPLANTED ISLETS, GREEN, INHIBITION, EXPRESSION, VECTORS, TUMORS, MODEL
  • Akdeniz Üniversitesi Adresli: Evet

Özet

Background To increase the success rate in xenogeneic islet transplantation, proper assessment of graft mass is required following transplantation. For this reason, we aimed to develop a suitable fluorescence imaging system to monitor islet xenograft survival in diabetic mice.

BACKGROUND: To increase the success rate in xenogeneic islet transplantation, proper assessment of graft mass is required following transplantation. For this reason, we aimed to develop a suitable fluorescence imaging system to monitor islet xenograft survival in diabetic mice.

METHODS:

Adenovirus vector encoding enhanced green fluorescent protein-transduced rat pancreatic islets were transplanted under the renal capsule of streptozotocin-induced diabetic mice and the fluorescence signal was quantified over time using a cooled charge-coupled device. Non-fasting blood glucose levels were recorded during the same period. Insulin release from transduced and control islets was detected via enzyme-linked immunosorbent assay.

RESULTS:

Adenovirus vector encoding enhanced green fluorescent protein infection did not alter the function or survival of pancreatic islets post transduction. A direct correlation was found between the number of islets (250-750) transplanted under the kidney capsule and the blood glucose recovery.

CONCLUSIONS:

Fluorescence imaging appears to be a useful tool for quantitative assessment of islet cell viability post transplantation and could permit earlier detection of graft rejection.