Tracing of islet graft survival by way of in vivo fluorescence imaging
DIABETES-METABOLISM RESEARCH AND REVIEWS, cilt.27, sa.6, ss.575-583, 2011 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 27 Sayı: 6
- Basım Tarihi: 2011
- Doi Numarası: 10.1002/dmrr.1216
- Dergi Adı: DIABETES-METABOLISM RESEARCH AND REVIEWS
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.575-583
- Anahtar Kelimeler: EGFP, in vivo imaging, adenovirus, type 1 diabetes, xenogeneic islet transplantation, TYPE-1 DIABETES-MELLITUS, LUNG-CANCER CELLS, ADENOVIRUS DELIVERY, TRANSPLANTED ISLETS, GREEN, INHIBITION, EXPRESSION, VECTORS, TUMORS, MODEL
- Akdeniz Üniversitesi Adresli: Evet
Özet
BACKGROUND: To increase the success rate in xenogeneic islet transplantation, proper assessment of graft mass is required following transplantation. For this reason, we aimed to develop a suitable fluorescence imaging system to monitor islet xenograft survival in diabetic mice.
METHODS:
Adenovirus vector encoding enhanced green fluorescent protein-transduced rat pancreatic islets were transplanted under the renal capsule of streptozotocin-induced diabetic mice and the fluorescence signal was quantified over time using a cooled charge-coupled device. Non-fasting blood glucose levels were recorded during the same period. Insulin release from transduced and control islets was detected via enzyme-linked immunosorbent assay.
RESULTS:
Adenovirus vector encoding enhanced green fluorescent protein infection did not alter the function or survival of pancreatic islets post transduction. A direct correlation was found between the number of islets (250-750) transplanted under the kidney capsule and the blood glucose recovery.
CONCLUSIONS:
Fluorescence imaging appears to be a useful tool for quantitative assessment of islet cell viability post transplantation and could permit earlier detection of graft rejection.