Cytotoxicity of temporary cements on bovine dental pulp-derived cells (bDPCs) using realtime cell analysis


Malkoç M., Demir N., Şengün A., Bozkurt Ş. B., Hakki S. S.

Journal of Advanced Prosthodontics, vol.7, no.1, pp.21-26, 2015 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 7 Issue: 1
  • Publication Date: 2015
  • Doi Number: 10.4047/jap.2015.7.1.21
  • Journal Name: Journal of Advanced Prosthodontics
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.21-26
  • Keywords: Cytotoxicity, Dental pulp-derived cells, Real-time cell analysis, Temporary cements
  • Akdeniz University Affiliated: No

Abstract

Purpose: To evaluate the cytotoxicity of temporary luting cements on bovine dental pulp-derived cells (bDPCs). Materials and methods: Four different temporary cements were tested: Rely X Temp E (3M ESPE), Ultratemp (Ultradent), GC Fuji Temp (GC), and Rely X Temp NE (3M ESPE). The materials were prepared as discs and incubated in Dulbecco's modified eagle's culture medium (DMEM) for 72 hours according to ISO 10993-5. A real-time cell analyzer was used to determine cell vitality. After seeding 200 μL of the cell suspensions into the wells of a 96-well plate, the bDPCs were cured with bioactive components released by the test materials and observed every 15 minutes for 98 hours. One-way ANOVA and Tukey-Kramer tests were used to analyze the results of the proliferation experiments. Results. All tested temporary cements showed significant decreases in the bDPCs index. Rely X Temp E, GC Fuji Temp, and Rely X Temp NE were severely toxic at both time points (24 and 72 hours) (P < .001). When the cells were exposed to media by Ultratemp, the cell viability was similar to that of the control at 24 hours (P > .05); however, the cell viability was significantly reduced at 72 hours (P < .001). Light and scanning electron microscopy examination confirmed these results. Conclusion: The cytotoxic effects of temporary cements on pulpal tissue should be evaluated when choosing cement for luting provisional restorations.