Nitrotyrosine formation and apoptosis in rat models of ocular injury

Aslan M., Yucel I., Akar Y., Yucel G., Ciftcioglu M., Sanlioglu S.

FREE RADICAL RESEARCH, cilt.40, sa.2, ss.147-153, 2006 (SCI-Expanded, Scopus)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 40 Sayı: 2
  • Basım Tarihi: 2006
  • Doi Numarası: 10.1080/10715760500456219
  • Dergi Adı: FREE RADICAL RESEARCH
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.147-153
  • Anahtar Kelimeler: nitrotyrosine, inducible nitric oxide synthase, apoptosis, intraocular pressure, lipopolysaccharide, NITRIC-OXIDE SYNTHASE, ENDOTOXIN-INDUCED UVEITIS, ELEVATED INTRAOCULAR-PRESSURE, CHRONIC EXPERIMENTAL GLAUCOMA, OPTIC-NERVE HEAD, TYROSINE NITRATION, AQUEOUS-HUMOR, CELLS, DISEASE, EXPRESSION
  • Akdeniz Üniversitesi Adresli: Evet

Özet

Nitrotyrosine formation and apoptosis in rat models of ocular injury.

Aslan M1, Yücel I, Akar Y, Yücel G, Ciftçioğlu MA, Sanlioglu S.

 

 

Abstract

This study was performed to examine inducible nitric oxide synthase (NOS-2) expression, nitrotyrosine formation and apoptosis in rats with elevated intraocular pressure (IOP) and/or ocular inflammation. Ocular inflammation was induced via injection of intra-vitreal lipopolysaccharide (LPS) while IOP was elevated by episcleral vessel cauterization. Animals were randomized to one of the following conditions: elevated IOP, LPS, elevated IOP+LPS, and control. Immunohistochemical staining and western blot analysis of retinal lysates revealed NOS-2 and nitrotyrosine immunoreactivity in all disease groups. NOS-2 expression and protein nitration was significantly greater in rats with elevated IOP+LPS compared to elevated IOP, LPS, and control groups. Nitrite levels in the retina affirmed significantly increased levels of nitric oxide generation in LPS-treated rats with elevated IOP (346+/-23.8 microM) vs LPS-treated, elevated IOP and control groups (195.6+/-12.6, 130+/-2.5 and 76.6+/-15.6 microM, respectively). Retinal TUNEL staining showed apoptosis in all diseased groups. Percent of apoptotic cells was significantly greater in the elevated IOP+LPS group compared to LPS-treated or elevated IOP groups. Presented data illustrates that both elevated IOP and ocular inflammation augment NOS-2 expression, retinal protein nitration and apoptosis in rats.