EVALUATION OF DNA DAMAGE IN CAPSICUM ANNUUM L. EXPOSED TO SPINOSAD IN THE COMET ASSAY

Demir E., KAYA N., KAYA B.

FRESENIUS ENVIRONMENTAL BULLETIN, cilt.20, sa.8, ss.1926-1930, 2011 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 20 Sayı: 8
  • Basım Tarihi: 2011
  • Dergi Adı: FRESENIUS ENVIRONMENTAL BULLETIN
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED)
  • Sayfa Sayıları: ss.1926-1930
  • Anahtar Kelimeler: Spinosad, Comet assay, Capsicum annuum, Genotoxicity, DNA damage, NATURALLY-DERIVED INSECTICIDE, CHRONIC TOXICITY, RISK-ASSESSMENT, VICIA-FABA, TOBACCO, CARCINOGENICITY, INDUCTION, MUTATION, DIPTERA, WORKERS
  • Akdeniz Üniversitesi Adresli: Evet

Özet

The naturally-derived insecticide Spinosad has been used to control thrips in Capsicum annuum L. Our aim was to determine the possible genotoxic effect of Spinosad in leaves C. annuum L. For this purpose, we used the Comet assay to investigate DNA damage in isolated cells from leaves of C. annuum L. in order to assess the potential hazards of Spinosad. For the evaluation of genotoxic effects, the % tail DNA (tail intensity) and the tail moment in the treated Spinosad were compared to the solvent control (distilled water). The alkaline Comet assay showed that both tail intensity and tail moment significantly increased at 0.5, 1, 5 and 10 ppm concentrations of Spinosad, meaning that Spinosad probably has apoptotic effects at a concentration of 10 ppm and higher. These results can contribute to increase the genotoxicity database on Spinosad.