Akademik Veri Yönetim Sistemi
Acta histochemica, cilt.109, sa.6, ss.468-79, 2007 (SCI-Expanded)
The aim of the study was to investigate the distribution and differentiation of cell types in the stroma of human placental. stem villi (SV). A total of 14 human term placental tissues were studied. Double immunolabeling was performed for desminvimentin, desmin-alpha-smooth actin and vimentin-alpha-smooth actin. Cytokeratin 7, proliferating cell nuclear antigen immunotabeling was also performed. Parallel. tissue samples were examined by transmission electron microscopy. HSCORE was performed for the semi-quantitative analysis of distribution of cells in the stroma of SV. Vimentinlabeled cells were mostly distributed in the subtrophoblastic area. Desmin-vimentin double immunolabeting was mainly localized in the triangular area and to a lesser degree in the perivascular area and vessel. watts (p = < 0.001). However, desmin-alpha smooth actin labeling was observed predominantly in the vessel watt and perivascular area. Vimentin-alpha. smooth actin immunoreactivity was significantly stronger in the triangular and perivascular areas compared to the vessel watts (p = 0.003). Ultrastructurally, cells in the stroma of SV were mesenchyme cells, reticulum cells, fibroblasts, myofibroblasts, smooth muscle cells, and Hofbauer cells, filamented and vacuolated cells. The differentiation of myofibroblasts in the triangular and perivascular areas may play a rote in maturation of SV and villous contractility, modulation of the intervillous space and this may have effects on maternofetal placental circulation. (c) 2007 Elsevier GmbH. All rights reserved.