Callus induction and plant regeneration from mature embryos of different wheat genotypes

Nasircilar A. G., Turgut K., Fiskin K.

PAKISTAN JOURNAL OF BOTANY, cilt.38, sa.3, ss.637-645, 2006 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 38 Sayı: 3
  • Basım Tarihi: 2006
  • Dergi Adı: PAKISTAN JOURNAL OF BOTANY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.637-645
  • Akdeniz Üniversitesi Adresli: Evet

Özet

Mature embryos of 5 Triticum aestivian and 5 T. durum cultivars formed embryogenic callus on two different media. Embryos were removed from surface sterilised seeds and placed with the scutellum upwards on a solid agar medium containing the inorganic components of Murashige & Skoog and 2 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) or 1 mg/L naphtalenacetic acid (NAA). The developed calli and regenerated plants were maintained on 2,4-D or NAA free MS medium. Wheat plants can be regenerated via two different systems. There were significant differences in percentage of callus induction and regeneration capacity on the different initiation medium. Among the T.aestivian cultivars, Yakar had the highest regeneration capacity in both induction medium. In T.durunz cultivars, Kiziltan gave the highest regeneration capacity in MS+2,4 D medium and Yilmaz gave the highest regeneration capacity in MS+NAA medium. A strong genotypic effect on the culture responses was found for both induction medium.