Akademik Veri Yönetim Sistemi
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, cilt.41, sa.9, ss.532-543, 2018 (SCI-Expanded)
Carvedilol is a drug used in the treatment of serious cardiovascular diseases. Therefore, the development of a new method for analyzing the therapeutic and toxic concentrations of carvedilol in human blood serum is very important. In this study, a simple, rapid, accurate, and sensitive method was developed using isocratic reversed phase-high performance liquid chromatography with diode array detector for the determination of carvedilol (a nonselective beta-blocker) in human blood serum. The separation was achieved with a Supelco Discovery C18 reversed phase column, using acetonitrile and 1% (v/v) of triethylamine aqueous solution (pH 3.5) as mobile phase. The method was optimized and subsequently validated. Method validation was performed by evaluating precision, accuracy, selectivity, linearity, limit of detection (LOD), limit of quantification (LOQ), stability, robustness, and system suitability parameters. Relative standard deviation (%) values for the repeatability study were very low. The calibration curve was found to be linear over the concentration range of 100-1000g/L (R-2=0.9999). The average recovery was obtained as 99.83 +/- 0.84%. The LOD and the LOQ for carvedilol was determined to be 56.63 and 171.61ng/L, respectively. The results obtained from this study demonstrated that the validated method can be successfully used to analyze the therapeutic and toxic concentrations of carvedilol in human blood serum. Furthermore, this new method is applicable for large sample series in laboratory studies.