Induction of xanthine oxidase activity, endoplasmic reticulum stress and caspase activation by sodium metabisulfite in rat liver and their attenuation by Ghrelin

Ercan S., Kencebay C., Basaranlar G., DERİN N., Aslan M.

FOOD AND CHEMICAL TOXICOLOGY, vol.76, pp.27-32, 2015 (SCI-Expanded, Scopus)

  • Publication Type: Article / Article
  • Volume: 76
  • Publication Date: 2015
  • Doi Number: 10.1016/j.fct.2014.11.021
  • Journal Name: FOOD AND CHEMICAL TOXICOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.27-32
  • Keywords: Sodium metabisulfite, Ghrelin, Endoplasmic reticulum stress, Xanthine oxidase, UNFOLDED PROTEIN RESPONSE, ANTIOXIDANT ENZYME-ACTIVITY, VISUAL-EVOKED POTENTIALS, INDUCED OXIDATIVE STRESS, SULFUR-DIOXIDE, LIPID-PEROXIDATION, SULFITE OXIDASE, CELL-DEATH, APOPTOSIS, DEHYDROGENASE
  • Akdeniz University Affiliated: Yes

Abstract

2014 Dec 5. pii: S0278-6915(14)00494-3. doi: 10.1016/j.fct.2014.11.021. [Epub ahead of print]

Induction of xanthine oxidase activity, endoplasmic reticulum stress and caspase activation by sodium metabisulfite in rat liver and their attenuation by ghrelin.

Ercan S1, Kencebay C2, Basaranlar G2, Derin N2, Aslan M3.

 

 

Abstract

Sodium metabisulfite is used as a preservative in many food preparations but can oxidize to sulfite radicals initiating molecular oxidation. Ghrelin is a peptide hormone primarily produced in the stomach and has anti-inflammatory and anti-oxidant effects on gastrointestinal and cardiovascular systems. This study was performed to elucidate the effect of ghrelin on sulfite-induced endoplasmic reticulum (ER) stress and caspase activation in rat peripheral organs. Xanthine oxidase (XO), xanthine dehydrogenase (XDH) enzyme activities, ER stress markers [phosphorylated PKR-like ER kinase (pPERK); C/EBP-homologous protein (CHOP)], caspase-3, -8 -9 activities, nuclear factor kappa-B (NF-kB) levels were determined in liver, heart and kidney of rats treated with sodium metabisulfite and/or ghrelin for 5 weeks. Sodium metabisulfite treatment significantly elevated XO activity, induced expression of GRP78, CHOP and increased caspase-3, -8 and -9 activities in liver but had no significant effect in heart and kidney. Ghrelin treatment decreased XO activity to baseline levels and attenuated ER stress and caspase activation in liver tissue of sodium metabisulfite treated rats. In conclusion, metabolism of sodium metabisulfite in liver tissue increased XO activity, induced ER stress and caused caspase activation which was attenuated by ghrelin treatment. Ghrelin's hepatoprotective effect could be through modulation of XO activity.

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KEYWORDS:

Sodium metabisulfite; endoplasmic reticulum stress; ghrelin; xanthine oxidase