Effect of different storage solutions on oxidative stress in human saphenous vein grafts

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Tekin I., Demir M., ÖZDEM S.

JOURNAL OF CARDIOTHORACIC SURGERY, cilt.17, sa.1, 2022 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 17 Sayı: 1
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1186/s13019-022-01752-7
  • Dergi Adı: JOURNAL OF CARDIOTHORACIC SURGERY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Agricultural & Environmental Science Database, CINAHL, EMBASE, MEDLINE, Directory of Open Access Journals
  • Anahtar Kelimeler: Vein graft failure, Coronary artery bypass graft, Storage solution, Endothelial damage inhibitor, FOLLOW-UP, ARTERY, PRESERVATION, OUTCOMES, FAILURE, PATENCY
  • Akdeniz Üniversitesi Adresli: Evet

Özet

Background Ischemia-reperfusion injury of saphenous vein grafts (SVG) during coronary artery bypass grafting surgery negatively impacts endothelial integrity and functionality and is associated with vein graft failure. The aim of this study was to evaluate the level of oxidative stress in human SVG segments following ischemic storage in three intraoperative graft storage solutions: saline (S), autologous heparinized blood (HB) and DuraGraft (DG). Methods 3 mm tissue rings derived from surplus SVG segments from 50 patients were stored at room temperature for 30 min in DG, S or HB. Total oxidative status (TOS) and total antioxidant status (TAS) levels were determined from which the oxidative stress index (OSI: TOS/TAS ratio) was calculated. A p-value < 0.017 was considered significant implementing a Bonferroni correction. Results TOS values were significantly lower for DG stored samples in comparison to both S and HB; there was no difference between S and HB (DG: 32.6 +/- 1.8, S: 39.6 +/- 2.8 and HB: 40.6 +/- 2.4 mu mol H2O2 eqv.; DG vs. S and DG vs. HB p < 0.0001, S vs. HB p = 0.047). TAS was higher for both DG and HB in comparison to S (DG: 8.9 +/- 0.9, S: 6.9 +/- 1.0 and HB: 8.6 +/- 0.9 mmol Trolox eqv.; DG vs S p < 0.0001, DG vs. HB p = 0.263, S vs. HB p < 0.0001). OSI differed between all groups with the lowest value for DG (DG: 3.7 +/- 0.2, S: 5.8 +/- 0.4 and HB: 4.7 +/- 0.2 mu mol H2O2 eqv./mmol Trolox eqv.; all p < 0.0001). Conclusions Saphenous veins grafts stored in DuraGraft had a lower oxidative level, higher antioxidant level and a lower oxidative stress index in comparison to saphenous vein grafts stored in saline or heparinized blood. ClinicalTrials.gov Identifier NCT02922088.