Akademik Veri Yönetim Sistemi
American Society for Reproductive Medicine (ASRM), Texas, Amerika Birleşik Devletleri, 28 Ekim - 01 Kasım 2017, ss.106-107, (Özet Bildiri)
DISTINCT SPATIOTEMPORAL EXPRESSION OF
FOXO1 IN PERIIMPLANTATION MOUSE UTERUS
AND REDUCED EMBRYO IMPLANTATION AFTER
ITS FUNCTIONAL BLOCKAGE. D. Adiguzel,a P. Sahin, a S. Ozkavukcu, b C. Celik-Ozenci. a a Histology and Embryology, Akdeniz University Faculty
of Medicine, Antalya, Turkey; b
Center for Assis- ted
Reproduction, Dep. of Obstetrics and Gynecology, Ankara University
School of Medicine, Ankara, Turkey.
OBJECTIVE: To investigate
uterine expression pattern and functional
regulation of FoxO1 during
peri-implantation period and to assess embryo
implantation dynamics when
FoxO1 is functionally blocked in mice.
DESIGN: Experimental mouse
models including; i) natural pregnancy, ii)
pseudopregnancy, iii)
artificial decidualization, and iv) pre-implantation
stage bioneutralization
(blockage) of the uterine FoxO1.
MATERIALS AND METHODS:
Uterine tissues of estrous phase and preg-
nancy (1-8 days) were
obtained from 6 weeks old BALB/C female mice. Expres-
sion of FoxO1 was
determined by immunohistochemistry (n¼6 in each group)
and western blotting (n¼3) during peri-implantation
period. Implantation sites
and embryo morphology were
evaluated after functional blockage of FoxO1
(n¼5). Group comparisons for
H-Score and western blot analyses were done
by one-way ANOVA followed by
the Tukey test employing GraphPad Prism 6
software. P values < 0.05 among different groups
were considered significant.
RESULTS: FoxO1 expression
was observed in a spatiotemporal manner in
peri-implantation uterus in
mice. Its expression in luminal and glandular epithe-
lium increased significantly
at the time of implantation (p<0.001). Number of
embryo implantation sites
decreased significantly after bioneutralization of uter-
ine FoxO1 at
pre-implantation stage (p< 0.0001). Evaluation of FoxO1 expres-
sion in pseudopregnancy and
artificial decidualization groups revealed that its
expression appeared to be
dependent on steroid hormones but independent
from the presence of the
blastocyst.
CONCLUSIONS: FoxO1 signaling
seems to be important for uterine
receptivity and implantation
in mice. Moreover, studies are ongoing in hu-
man regarding the evaluation
of expression patterns of FoxO1 in pre-recep-
tive and receptive
endometrium in our laboratory. A better understanding of
the molecular basis
governing both early pregnancy and uterine receptivity
will help to improve the
outcome of natural pregnancy and pregnancy
conceived via assisted
reproductive techniques.
Supported by: This project was supported by TUBITAK
(project number:
215S868)