Resazurin microplate test method for rapid determination of colistin resistance in carbapenem-resistant Acinetobacter baumanii isolates

Yildirim K., Simsek E., Kocak O., Bozkurt S., Koyuncu Ozyurt Ö., Coban A. Y.

Lab Medicine, cilt.55, sa.3, ss.380-385, 2024 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 55 Sayı: 3
  • Basım Tarihi: 2024
  • Doi Numarası: 10.1093/labmed/lmad099
  • Dergi Adı: Lab Medicine
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, CAB Abstracts, CINAHL
  • Sayfa Sayıları: ss.380-385
  • Anahtar Kelimeler: Acinetobacter baumannii, carbapenem resistance, colistin, colorimetric test, rapid antibiogram susceptibility test, resazurin
  • Akdeniz Üniversitesi Adresli: Evet

Özet

Background: Colistin-resistant Acinetobacter baumannii isolates are extremely important pathogens for hospital-Acquired infections. Objective: To investigate the effectiveness of the resazurin microplate assay (REMA) for the rapid determination of colistin resistance. Methods: Susceptibility for colistin was investigated in vitro by the broth microdilution method (BMD) and the resazurin microplate assay (REMA) on 106 carbapenem-resistant Acinetobacter baumannii isolates. Results: The results of both test methods were compared, and the categorical agreement between them was found to be 100%. No minor, major, or very major discrepancy was observed between the 2 methods. Conclusions: The most important advantages of REMA are that the results are obtained within 6 hours compared to the reference method, that it is easy to evaluate because it is colorimetric, and that the susceptibility result can be reported to the clinician on the same day as bacterial identification.