Akademik Veri Yönetim Sistemi
APPLIED ECOLOGY AND ENVIRONMENTAL RESEARCH, cilt.16, sa.5, ss.6835-6843, 2018 (SCI-Expanded)
The preliminary aim of the study before future breeding program was to reveal usability of ISSR markers to assess genetic diversity and relatedness within raspberry genotypes wildly grown in Northern Turkey. In this study, 19 wild raspberry genotypes collected from Black Sea region in Turkey were used. ISSR PCR analyses were carried on the DNA of genotypes isolated using mini CTAB extraction method. The levels of polymorphism between genotypes were determined using the UBC ISSR primers. A total of 111 bands were obtained from 15 UBC ISSR primers. Out of 111 bands, 101 bands were polymorphic. The number of bands obtained per primer ranged between 3 and 10, the average number of bands were determined as 7.4. The average number of polymorphic bands per primer was 6.73. Similarities and differences between genotypes have been studied at the molecular level. The data used for statistical analysis were obtained by the evaluation of ISSR bands. Similarity coefficient and UPGMA dendrogram were built using the Basic Coordinates Analysis. According to the dendrogram, the genotypes have been divided in two main groups, one small and other large group. While the small group was only comprised of three (A8, A10 and A14) genotypes, the large group included the rest of the genotypes studied.