Akademik Veri Yönetim Sistemi
V. INTERNATIONAL AGRICULTURAL, BIOLOGICAL &LIFE SCIENCECONFERENCE, Edirne, Türkiye, 17 - 20 Eylül 2023, ss.220
Sorghum (Sorghum bicolor (L.) Moench) is a diploid C4 cereal crop which is predominantly self-pollinated. It is a model crop with multiple uses, including food, feed and fuel. Sorghum was domesticated in Africa and it is currently the fifth major cereal produced in the world. In sorghum breeding, the use of molecular markers for genetic analysis and manipulation of agronomic traits is an increasingly useful tool for sorghum improvement. They are frequently used in the genotyping of plant material to map QTLs and evaluate population diversity. Molecular markers based on insertion-deletion (InDel) are currently the most widely used polymorphisms, known for their codominance and genome-wide distribution. They are easy to use, PCR-based and suitable for low-cost gel-based genotyping. From this perspective, this study was aimed to develop and validate InDel markers by bioinformatics analysis of DNA sequencing data associated with the double digestion restriction site (ddRADSeq) of 11 sorghum genotypes from different origins. DNA isolation was performed with the CTAB protocol and the resulting sequences were used for analysis. All 47 InDel sites with length ≥ 10 bp were developed. To validate these InDels, a total of 19 InDel regions were selected and primers were successfully amplified. After amplification, 16 genotypes were used in validation. This research shows that sequencing technologies can facilitate the development of genome-wide molecular markers. These agarose-resolvable InDel markers are expected to be useful for sorghum breeding studies in the future.