Sensitivity enhancement for microbial biosensors through cell Self-Coating with polypyrrole

Apetrei R., carac G., BAHRIM G., ÇAMURLU P.

INTERNATIONAL JOURNAL OF POLYMERIC MATERIALS AND POLYMERIC BIOMATERIALS, vol.68, no.17, pp.1058-1067, 2019 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 68 Issue: 17
  • Publication Date: 2019
  • Doi Number: 10.1080/00914037.2018.1525548
  • Journal Name: INTERNATIONAL JOURNAL OF POLYMERIC MATERIALS AND POLYMERIC BIOMATERIALS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.1058-1067
  • Keywords: Amperometric glucose detection, Aspergillus niger, In situ biosynthesis, Polypyrrole, Ppy-microbial biosensor, GLUCOSE-OXIDASE, CONDUCTING-POLYMER, YEAST-CELLS, GOLD NANOPARTICLES, IMMOBILIZATION, ENZYME, ELECTRODE, POLYANILINE, BIOCOMPATIBILITY, ENTRAPMENT
  • Akdeniz University Affiliated: Yes

Abstract

The development of amperometric glucose biosensors based on Aspergillus niger is an established method as is the use of conducting polymers (CPs) for improvement of analytical features. The novelty proposed herein consists in the synthesis of polypyrrole (Ppy) in situ, within the cellular membrane/wall for enhancement of electrochemical features of the cells in the context of electroanalytical applications. The process accomplishes a two-fold purpose: (i) it makes use of biocatalysts yielded by the cells without need for enzyme separation and purification; (ii) it involves cellular components, which perform self-coating with Ppy without need for chemical functionalization.