Identification of resistance gene analogs in cotton (Gossypium hirsutum L.)

TAN H., CALLAHAN F., ZHANG X., Karaca M., SAHA S., JENKINS J., ...Daha Fazla

EUPHYTICA, cilt.134, sa.1, ss.1-7, 2003 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 134 Sayı: 1
  • Basım Tarihi: 2003
  • Doi Numarası: 10.1023/a:1026114327168
  • Dergi Adı: EUPHYTICA
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1-7
  • Anahtar Kelimeler: cotton, gene markers, nucleotide binding site, polymerase chain reaction, resistance gene analogs, PLANT-DISEASE RESISTANCE, LEUCINE-RICH REPEAT, NUCLEOTIDE-BINDING SITE, ROOT-KNOT NEMATODE, RUST RESISTANCE, ARABIDOPSIS-THALIANA, MICROBE INTERACTIONS, HOMOLOGS, SUPERFAMILY, PROTEIN
  • Akdeniz Üniversitesi Adresli: Evet

Özet

Sequence analyses of numerous plant disease resistance genes have revealed the presence of conserved motifs common to this class of genes, namely a nucleotide binding site (NBS) and leucine rich repeat region. In this study, thirty-three resistance gene analogs (RGAs) were cloned and sequenced from cotton (Gossypium hirsutum L.) following PCR with degenerate primers designed from the conserved NBS motif of plant resistance (R) genes. Phylogenetic analysis of the predicted amino acid sequences grouped the RGAs into four distinct classes from which several subgroups were delineated based on nucleic acid sequences. Gene database searches with the consensus protein sequences of each of the four classes and respective subgroups of cotton RGAs revealed their conserved NBS domains and homology to RGAs and known resistance genes from a variety of plant genera. Given the complete lack of knowledge regarding molecular organization of R genes in cotton, the cloned RGAs described here may be useful as probes to map, characterize, and manipulate R genes of the cotton genome.