Increased resistance to oxidative stress in normal and glucose-6-phosphate dehydrogenase-deficient hemolysates in the presence of enzyme substrates

Yucel G., Yesilkaya A., Aksu T., Yegin A., Ahciguzel Y.

INTERNATIONAL JOURNAL OF CLINICAL & LABORATORY RESEARCH, vol.27, no.1, pp.55-59, 1997 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 27 Issue: 1
  • Publication Date: 1997
  • Doi Number: 10.1007/bf02827243
  • Journal Name: INTERNATIONAL JOURNAL OF CLINICAL & LABORATORY RESEARCH
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.55-59
  • Keywords: glucose-6-phosphate dehydrogenase-deficiency, lipid peroxidation, oxidative stress, chemiluminescence, HUMAN RED-CELLS, LIPID-PEROXIDATION, HYDROPEROXIDE, ERYTHROCYTES, CHEMILUMINESCENCE, OXYGEN
  • Akdeniz University Affiliated: Yes

Abstract

Erythrocytes and hemolysates from 10 normal and 10 glucose-6-phosphate dehydrogenase-deficient individuals were incubated with cumene hydroperoxide, and free radical-induced lipid peroxidation was monitored by chemiluminescence. Chemiluminescence intensities in erythrocytes of normal and deficient subjects were similar in the presence or absence of glucose-6-phosphate dehydrogenase substrates. Hemolysates of normal and deficient subjects also showed similar chemiluminescence in the absence of substrates. However, with the addition of substrates to the incubation medium, deficient hemolysates reached maximum chemiluminescence intensity within a shorter period, and maximum values were higher than in normal hemolysates. We believe this offers a new means of detection of glucose-6-phosphate dehydrogenase-deficient patients.