Increased resistance to oxidative stress in normal and glucose-6-phosphate dehydrogenase-deficient hemolysates in the presence of enzyme substrates

Yucel G., Yesilkaya A., Aksu T., Yegin A., Ahciguzel Y.

INTERNATIONAL JOURNAL OF CLINICAL & LABORATORY RESEARCH, cilt.27, sa.1, ss.55-59, 1997 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 27 Sayı: 1
  • Basım Tarihi: 1997
  • Doi Numarası: 10.1007/bf02827243
  • Dergi Adı: INTERNATIONAL JOURNAL OF CLINICAL & LABORATORY RESEARCH
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.55-59
  • Anahtar Kelimeler: glucose-6-phosphate dehydrogenase-deficiency, lipid peroxidation, oxidative stress, chemiluminescence, HUMAN RED-CELLS, LIPID-PEROXIDATION, HYDROPEROXIDE, ERYTHROCYTES, CHEMILUMINESCENCE, OXYGEN
  • Akdeniz Üniversitesi Adresli: Evet

Özet

Erythrocytes and hemolysates from 10 normal and 10 glucose-6-phosphate dehydrogenase-deficient individuals were incubated with cumene hydroperoxide, and free radical-induced lipid peroxidation was monitored by chemiluminescence. Chemiluminescence intensities in erythrocytes of normal and deficient subjects were similar in the presence or absence of glucose-6-phosphate dehydrogenase substrates. Hemolysates of normal and deficient subjects also showed similar chemiluminescence in the absence of substrates. However, with the addition of substrates to the incubation medium, deficient hemolysates reached maximum chemiluminescence intensity within a shorter period, and maximum values were higher than in normal hemolysates. We believe this offers a new means of detection of glucose-6-phosphate dehydrogenase-deficient patients.