Structural assesment of phosphopeptide enrichment potential of the newly developed lanthanide(III) ions based magnetic affinity materials

Topel Ö., Gök V., Aksu S., Persson I.

NanoTR-17, İzmir, Türkiye, 27 - 29 Ağustos 2023, ss.38

  • Yayın Türü: Bildiri / Özet Bildiri
  • Basıldığı Şehir: İzmir
  • Basıldığı Ülke: Türkiye
  • Sayfa Sayıları: ss.38
  • Akdeniz Üniversitesi Adresli: Evet

Özet

It has been developed a new lanthanide(III) ion-based magnetic IMAC materials (Ln3+-Mag-IMAC) consisting

of core–shell–like silica-coated magnetic nanoparticles as supporting materials, chelidamic acid as a chelating

agent, and Ln3+ ions as metal ions [1]. The potential of the developed Ln3+-Mag-IMAC materials in the

enrichment of phosphopeptides was modeled by phosphate adsorption studies in an aqueous solution at 25o

C. The results revealed a good phosphate adsorption capacity, with a stoichiometry of 2.5, 1.2, and 1.1 moles of

phosphate ions per mole of Nd3+, Dy3+, and Er3+ ions on the surface of Ln3+-Mag-IMAC materials. Structural

assessments using Extended X-ray Absorption Fine Structure (EXAFS) spectroscopy measurements indicated

that Nd3+ ions on the surface of Ln3+-Mag-IMAC materials exhibit a nine-coordinate structure, allowing for

three possible phosphate coordination sites within the first coordination sphere; but, Dy3+ and Er3+ ions with a

seven-coordinate configuration on the IMAC surface possess the ability to accommodate only two phosphate

ions. The developed Ln3+-Mag-IMAC materials were applied on trypsin-digested beta-casein solutions, and

the captured phosphopeptides were identified by LC-MS/MS analysis.