Effect of replacing dietary fish meal with poultry by-product meal on liver histology of common carp fry (Cyprinus carpio).


AYDIN B., BALCI B. A., Aktop Y., GÜMÜŞ E., Aksoy A.

FABA 2014 International Symposium on Fisheries and Aquatic Seciences, Trabzon, Turkey, 25 - 27 September 2014, pp.278

  • Publication Type: Conference Paper / Summary Text
  • City: Trabzon
  • Country: Turkey
  • Page Numbers: pp.278
  • Akdeniz University Affiliated: Yes

Abstract

Objective: The aim of this study was to investigate the effect of graded levels of poultry by-product meal (PBM) in diets on histology of liver in common carp fry.

Methods: Triplicate groups of fish were fed each of five isoproteic (34% crude protein), isolipidic (9% crude lipid) and isoenergetic (3750 kcal DE g-1) diets formulated to contain PBM replaced 0, 25, 50, 75, and 100% of the protein supplied by fish meal. 20 fish were placed in each of the aquarium (65 L) and fed manually to apparent satiation with one of experimental diets twice daily for 13 weeks. For histological investigations tissue samples from livers were collected and fixed in 10% buffered neutral formalin solution. After the routine alcohol-xylol process, tissue samples were embedded in paraffin and sectioned in 5μm thick pieces using rotary microtome. All sections were stained with hematoxylen and eosine and investigated under the light microscopy.

Results and Discussion: The histological examinations of liver tissue samples of the common carp fed PBM-based diets revealed no differences. Liver tissue samples were generally normal but low level of lipid vacuolization was spotted in the cytoplasts of hepatocytes.

Key Words: Cyprinus carpio, fish meal, poultry by-product meal,  liver histology