Akademik Veri Yönetim Sistemi
3rd Annual International Conference on Public Health, 19-22 June 2017, Athens, Greece, Atina, Yunanistan, 19 - 22 Haziran 2017, ss.32-33
Amyloid beta (Ab) is the principal component of the amyloid plaques and initiate pathological processes in the cortex, hippocampus, and several other structures of the brain in the Alzheimer’s disease (AD). Therefore, we aimed to identify how brain electrical activity change by Ab injection and whether the observed changes can be used as a new biomarker for early diagnosis of the AD.
In our present study, 64 Wistar rats were divided randomly into eight experimental groups: Control group (C) and seven AD model groups obtained by intracerebroventricular Ab42 injection in increasing concentrations. In all rats, anesthesia was provided with mixture of Ketamine-based anesthetics (Ketamine, 50 mg/kg and Xylazine, 10 mg/kg; intraperitoneally, i.p.). Under the anesthesia, either Ab42 peptide or saline (0.9% NaCl) was injected into both lateral ventricles in 1 ml/minute rate by using Hamilton microsyringe. Following this process, recording electrodes were placed bilaterally into the frontal, parietal, temporal and occipital cortex while the reference electrode was placed into cerebellum. Then, auditory evoked potentials (AEPs) were recorded by using multi-electrodes in awake, freely moving rats, and were analyzed mathematically to determine the changes related to accumulation of Ab.
There was no difference in the latency values of AEPs between groups, but injection of Ab42 significantly affected peak to peak amplitudes of AEPs. It was also found that there were significant differences among the auditory evoked gamma responses between groups.
These results demonstrated that Aβ administration induces significant changes in brain activity and the observed changes in the gamma responses could be used to determine the localization of the Aβ in the brain.
This work was supported by the Scientific and Technological Research Council of Turkey (TUBITAK) (SBAG 315S054)