ANTIGENOTOXIC EFFECTS OF RESVERATROL IN SOMATIC CELLS OF DROSOPHILA MELANOGASTER

Turna F., Aksakal S., DEMIR E., KAYA B.

FRESENIUS ENVIRONMENTAL BULLETIN, cilt.23, sa.9, ss.2116-2125, 2014 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 23 Sayı: 9
  • Basım Tarihi: 2014
  • Dergi Adı: FRESENIUS ENVIRONMENTAL BULLETIN
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.2116-2125
  • Anahtar Kelimeler: Resveratrol, antigenotoxicity, ethyl methane sulfonate, potassium dichromate, Drosophila wing spot test, alkaline Comet assay, IN-VIVO GENOTOXICITY, CANCER CHEMOPREVENTIVE ACTIVITY, DNA-DAMAGE, HEXAVALENT CHROMIUM, HYDROGEN-PEROXIDE, OXIDATIVE STRESS, COMET ASSAY, LIFE-SPAN, MUTATION, MUTAGENICITY
  • Akdeniz Üniversitesi Adresli: Evet

Özet

The resveratrol (RSV, 3,4',5-trihydroxy stilbene) is a phytoalexin which is naturally occurring in different plants. The in vivo antigenotoxic effects of RSV against ethyl methane sulfonate (EMS) and potassium dichromate (K2Cr2O7) which causes genotoxicity with different mechanisms was studied in Drosophila melanogaster by using the Drosophila SMART assay and the alkaline Comet assay. For SMART assay the larvae were treated both pre-treatments and co-treatments, for Comet assay the larvae were treated simultaneous exposure. The results from the SMART assay showed that in pre-treatments and co-treatments of RSV with EMS and K2Cr2O7 was reduced the genotoxicity in all concentrations excluding only 1 mM concentration in co-treatments of RSV with EMS. Antigenotoxic effects of RSV against EMS and K2Cr2O7 is higher in pretreatment compared to co-treatment and stronger in especially against genotoxic damage caused by K2Cr2O7. In results of Comet assay RSV showed antigenotoxic effects against genotoxic damage caused by EMS and K2Cr2O7 for tail intensity (%) and tail moment (mu m). These results confirm the usefulness of the Comet assay with haemocytes as an in vivo model and support the possibility of antigenotoxicity associated with RSV. This is the first study reporting antigenotoxicity data in somatic cells of Drosophila for RSV.