Effect of storage on the respirometric relationship between substrate utilization and microbial growth

ÇIĞGIN A. S., Orhon D.

BIORESOURCE TECHNOLOGY, cilt.167, ss.331-335, 2014 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 167
  • Basım Tarihi: 2014
  • Doi Numarası: 10.1016/j.biortech.2014.06.039
  • Dergi Adı: BIORESOURCE TECHNOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.331-335
  • Anahtar Kelimeler: Sequencing batch reactor, Substrate storage, Polyhydroxybutyrate (PHB), Oxygen uptake rate, Process stoichiometry, BETA-HYDROXYBUTYRATE METABOLISM, SEQUENCING BATCH REACTOR, ACTIVATED-SLUDGE, PHB, STOICHIOMETRY, KINETICS, WASTES
  • Akdeniz Üniversitesi Adresli: Evet

Özet

The paper evaluated the impact of substrate storage on the respirometric assessment of process stoichiometry based on oxygen uptake rate (OUR) measurements. Two parallel sequencing batch reactors were operated with pulse feeding of synthetic substrate mixture at a sludge age of 8 days and 2 days. During the cycle experiments with acetate, 40-45% of acetate was converted to polyhydroxybutyrate, which was partly consumed during each cycle. Respirometric analysis also yielded OUR profiles for the corresponding cyclic operation. A mass balance expression was derived based on oxygen utilization. Oxygen demands calculated on the basis of partial PHB utilization closely matched the experimental values retrieved from OUR profiles within limits of analytical precision. The relative contribution of storage mechanism represented more than 50% of overall oxygen demand. Substrate storage, when totally disregarded or not properly evaluated, was observed to involve an error of around 10% on overall the oxygen demand. (C) 2014 Elsevier Ltd. All rights reserved.