Akademik Veri Yönetim Sistemi
Turkish Journal of Technology and Applied Sciences, cilt.6, sa.2, ss.101-110, 2025 (Hakemli Dergi)
Lettuce (Lactuca sativa L.) is an economically and nutritionally important vegetable crop cultivated worldwide. However, its susceptibility to various biotic and abiotic stresses necessitates genetic improvement through biotechnological tools. Agrobacterium tumefaciens-mediated transformation is a key method in plant biotechnology and plays a vital role in genetic modification studies in lettuce. This study aimed to compare transformation efficiency among different lettuce varieties and Agrobacterium strains. Cotyledon explants from nine lettuce varieties (Caipira, Emocion, Festival, Funtime, Garone, Maritima, Ruzgar, Surpriz, and Vaidosa) were subjected to transformation using two Agrobacterium strains, LBA4404 and EHA105. Both strains carried a binary vector containing a selectable marker and CRISPR-related elements. This vector was not used for gene editing but to assess delivery efficiency of a large-sized plasmid. Following transformation, explants were cultured on phosphinothricin (PPT)-selective MS medium. The highest shoot regeneration was observed in Emocion and Caipira. PCR analysis confirmed successful transgene integration in regenerated plants. Statistical analysis indicated that EHA105 provided significantly higher transformation efficiency than LBA4404 (p < 0.05). Among the tested genotypes, Caipira produced the highest number of transgenic shoots and showed superior responsiveness. Additionally, the region near the petiole on the cotyledon was found to be more favorable for shoot induction. This study contributes to protocol optimization for enhancing transformation efficiency in lettuce based on genotype and Agrobacterium strain selection.