The effect of CYP1A2 gene polymorphisms on Theophylline metabolism and chronic obstructive pulmonary disease in Turkish patients


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USLU A., OGUS C., ÖZDEMİR T., BİLGEN T., TOSUN O., KESER İ.

BMB REPORTS, vol.43, no.8, pp.530-534, 2010 (SCI-Expanded, Scopus)

  • Publication Type: Article / Article
  • Volume: 43 Issue: 8
  • Publication Date: 2010
  • Doi Number: 10.5483/bmbrep.2010.43.8.530
  • Journal Name: BMB REPORTS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.530-534
  • Keywords: COPD, Polymorphism, P450(CYP)1A2, Theophylline, Turkish population, 5'-FLANKING REGION, PHENOTYPE, GENOTYPE, ASSOCIATION
  • Open Archive Collection: AVESIS Open Access Collection
  • Akdeniz University Affiliated: Yes

Abstract

Cytochrome P450 (CYP) 1A2 gene polymorphisms are thought to be involved in the metabolism of theophylline (TP). We aimed to investigate the effect of CYP1A2*1C, CYP1A2*1D, CYP1A2*1E, and CYP1A2*1F polymorphisms of the CYP1A2 on TP metabolism by PCR-RFLP in 100 Turkish patients with chronic obstructive pulmonary disease (COPD) receiving TP. One hundred and one healthy volunteers were included as control group. The genotype frequencies of the CYP1A2*1D and CYP1A2*1F were found to be significantly different in the patients compared to the controls. The "T" allele at -2467 delT and the "C" allele at -163 C > A in the CYP1A2 displayed association with a significantly increased risk for COPD. "T" allele at -2467 delT was also associated with a high risk of disease severity in COPD. In conclusion, our data suggest that genetic alterations in CYP1A2 may play a role both in the pharmacogenetics of TP and in the development of COPD. [BMB reports 2010; 43(8): 530-534]