INVESTIGATION OF THE EFFECTS OF COBALT CHLORIDE AND POTASSIUM DICHROMATE ON THE GENOTOXIC ACTIVITY AND THE LONGEVITY OF DROSOPHILA MELANOGASTER

Demir E.

FRESENIUS ENVIRONMENTAL BULLETIN, vol.21, no.6, pp.1403-1409, 2012 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 21 Issue: 6
  • Publication Date: 2012
  • Journal Name: FRESENIUS ENVIRONMENTAL BULLETIN
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.1403-1409
  • Keywords: Longevity, Cobalt chloride, Potassium dichromate, Genotoxicity, SMART, Drosophila melanogaster, WING SPOT-TEST, CHROMIUM(VI) OXIDE, SOMATIC MUTATION, IN-VIVO, METAL, CARCINOGENICITY, MUTAGENICITY, CHLOROPHYLLIN, REDUCTION, DAMAGE
  • Akdeniz University Affiliated: Yes

Abstract

Metals are important environmental pollutants. Cobalt chloride (CoCl2) and potassium dichromate (K2Cr2O7) are widely distributed in both terrestrial and aquatic environments. Via water and soil may enter the food chain. Therefore, human are exposed, include consumer products and industrial wastes. Many organ systems are affected by metals which target specific biochemical processes and membranes of organelles and cells. For this purpose, as two metals namely, CoCl2 and K2Cr2O7 were evaluated for longevity on the wild-type strain Oregon-R+ of Drosophila and genotoxic effects in the somatic mutation and recombination tests (SMART) of Drosophila melanogaster following standard procedures. The wild-type strain Oregon-R+ of D. melanogaster was used as a test strain for the effect of longevity. The SMART assay in D. melanogaster has been designed to detect the genotoxic damages in a rapid an inexpensive way in one generation. Importance of SMART assay is to be in in vivo system and the metabolic machinery of Drosophila cells similar to mammalian cells. Both CoCl2, and K2Cr2O7 clearly significant a dose-dependent decrease the incidence of longevity while showed significant a dose-dependent increase to genotoxic activity in Drosophila. These in vivo results contribute to increase the genotoxicity and longevity database on the CoCl2 and K2Cr2O7.