Non-repressing carbon sources for alcohol oxidase (AOX1) promoter of Pichia pastoris

Inan M., Meagher M.

JOURNAL OF BIOSCIENCE AND BIOENGINEERING, vol.92, no.6, pp.585-589, 2001 (SCI-Expanded, Scopus) identifier identifier

  • Publication Type: Article / Article
  • Volume: 92 Issue: 6
  • Publication Date: 2001
  • Doi Number: 10.1263/jbb.92.585
  • Journal Name: JOURNAL OF BIOSCIENCE AND BIOENGINEERING
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.585-589
  • Keywords: Pichia pastoris, carbon catabolite repression, AOX1 promoter, CATABOLITE REPRESSION, PROTEIN-PRODUCTION, GENE-EXPRESSION, METHANOL, YEAST, FERMENTATION, METABOLISM, CULTURES, GLUCOSE, LACZ
  • Akdeniz University Affiliated: No

Abstract

The growth of Pichia pastoris in a mixture of either glycerol or glucose and methanol follows a diauxic growth, with C-1 utilizing enzymes being repressed. Therefore, these carbon sources can not be used as a mixture with methanol to simultaneously grow P. pastoris and induce C-1 utilizing enzymes, especially in a shake flask cultures of AOX-deficient P. pastoris. Among the alternative carbon sources tested, alanine, sorbitol, mannitol and trehalose, did not repress beta-gal production when methanol was used as an inducer in mut(-) strain of P. pastoris. Our results show that either one of alanine, sorbitol, mannitol or trehalose can be used as a sole carbon and energy source for P. pastoris, although the doubling time on trehalose was very long. Mut(-) strains growing in media containing trehalose, alanine, sorbitol and mannitol with methanol (0.5%) as an inducing agent expressed as much or higher amount of beta-gal as compared to the mut(+) growing in methanol containing media.