Development of a reversed phase-high performance liquid chromatographic method for the analysis of glucosamine sulphate in dietary supplement tablets

SEMIZ A., DUMAN O., TUNÇ S.

JOURNAL OF FOOD COMPOSITION AND ANALYSIS, cilt.93, 2020 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 93
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1016/j.jfca.2020.103607
  • Dergi Adı: JOURNAL OF FOOD COMPOSITION AND ANALYSIS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, PASCAL, Analytical Abstracts, BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Food Science & Technology Abstracts, Veterinary Science Database
  • Anahtar Kelimeler: Glucosamine sulphate, Analysis, Derivatization, RP-HPLC-DAD, Method validation, Dietary supplement tablet, KNEE OSTEOARTHRITIS, RAW-MATERIALS, CHONDROITIN, EFFICACY
  • Akdeniz Üniversitesi Adresli: Evet

Özet

Glucosamine sulphate can be taken orally from the dietary supplement tablets for the treatment of osteoarthritis. In these tablets, the determination of glucosamine sulphate concentration is very important for the identification of fraudulent products and quality-control studies. In this study, a simple, rapid, accurate and sensitive method was developed for the determination of glucosamine sulphate in the dietary supplement tablets by using isocratic reversed-phase high-performance liquid chromatography with diode array detector (RP-HPLC-DAD). The separation process was performed by Prodigy (TM) C18 reversed-phase column using an acetonitrile:H2O mixture (50:50 (v/v), pH 4.0) as mobile phase. The method developed was optimized and subsequently validated. Method validation was performed to illustrate the reliability of new analytical method through various validation parameters including linearity, precision, accuracy, limit of detection (LOD), limit of quantification (LOQ), selectivity and stability. Relative standard deviation (%) values for the intra-day and inter-day precision studies were low. The calibration curve was linear in the glucosamine sulphate concentration range of 0.05-2.5 mg.L-1. In this method, recovery values were obtained in the range between 85.15% and 118.86% and LOD and LOQ values were determined to be 0.014 mg.L-1 and 0.046 mg.L-1, respectively. Furthermore, all results confirmed that the new method is suitable for the determination of glucosamine sulphate in dietary supplement tablets.