The effect of quercetin on topotecan cytotoxicity in MCF-7 and MDA-MB 231 human breast cancer cells

Akbas S. H., Timur M., Ozben T.

JOURNAL OF SURGICAL RESEARCH, vol.125, no.1, pp.49-55, 2005 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 125 Issue: 1
  • Publication Date: 2005
  • Doi Number: 10.1016/j.jss.2004.11.011
  • Journal Name: JOURNAL OF SURGICAL RESEARCH
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.49-55
  • Keywords: breast cancer, Topotecan, ROS, nitrite, Quercetin, ESTROGEN-RECEPTOR, P-GLYCOPROTEIN, DRUG, PROLIFERATION, APOPTOSIS, ASSAY, INHIBITION, EXPRESSION, FLAVONOIDS, PEROXIDE
  • Akdeniz University Affiliated: Yes

Abstract

Background. Topotecan, which is a Camptothecin derivative, shows a large spectrum in anti-tumor activity. Topotecan exerts its cytotoxic effect on tumor cells mainly by inhibition of topoisomerase I activity resulting in double-strand DNA breaks. In our study, we investigated the combined cytotoxic action of Topotecan and Quercetin in MCF-7 and MDA-MB 231 human breast cancer cells. To examine the possible relation between the cytotoxic activity of Topotecan and oxidative stress, we measured ROS and nitrite levels in both human breast cell lines.