Increased expression of nestin in the major pelvic ganglion following cavernous nerve injury

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Kutlu O., Ross A. E., Schaeffer E. M., GRATZKE C., STIEF C. G., Strong T. D., ...Daha Fazla

INTERNATIONAL JOURNAL OF IMPOTENCE RESEARCH, cilt.24, sa.2, ss.84-90, 2012 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 24 Sayı: 2
  • Basım Tarihi: 2012
  • Doi Numarası: 10.1038/ijir.2011.50
  • Dergi Adı: INTERNATIONAL JOURNAL OF IMPOTENCE RESEARCH
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.84-90
  • Anahtar Kelimeler: erectile dysfunction, prostate cancer, radical prostatectomy, stem cell, FILAMENT PROTEIN NESTIN, FOCAL CEREBRAL-ISCHEMIA, MESENCHYMAL STEM-CELLS, NITRIC-OXIDE SYNTHASE, INTERMEDIATE-FILAMENT, ERECTILE FUNCTION, SPINAL-CORD, RAT, PROSTATECTOMY, MARKERS
  • Akdeniz Üniversitesi Adresli: Hayır

Özet

In an effort to identify neuronal repair mechanisms of the major pelvic ganglion (MPG), we evaluated changes in the expression of nestin, an intermediate filament protein and neural stem cell marker following cavernous nerve crush injury (CNI). We utilized two groups of Sprague Dawley rats: (i) sham and (ii) bilateral CNI. Erectile responses to cavernous nerve stimulation (CNS) were determined at 48h in a subset of rats. The MPG was isolated and removed at 48h after CNI, and nestin immunolocalization, protein levels and RNA expression were evaluated. At 48 h, erectile responses to CNS in CNI rats were substantially reduced (P<0.05; similar to 70% decrease in intra-cavernous pressure/mean arterial pressure) compared with sham surgery controls. This coincided with a dramatic 10-fold increase (P<0.05) in nestin messenger RNA expression and protein levels in the MPG of rats with CNI. Immunoflourescence microscopy demonstrated that nestin upregulation after CNI occurred within the ganglion cell bodies and nerve fibers of the MPG. In conclusion, CNI induces nestin in the MPG. These data suggest that nestin may be involved in the regenerative process of the cavernous nerve following crush injury. International Journal of Impotence Research (2012) 24, 84-90; doi:10.1038/ijir.2011.50; published online 13 October 2011