Akademik Veri Yönetim Sistemi
APPLIED ECOLOGY AND ENVIRONMENTAL RESEARCH, cilt.17, sa.6, ss.14927-14944, 2019 (SCI-Expanded)
Olive knot disease caused by Pseudomonas savastanoi pv. savastanoi (Psv) is one of the major diseases influencing olive (Oleae europaea L.) production in Turkey. The disease incidence rate was found to range between 4 and 80% according to the 2015 and 2018 surveys. A total of 67 isolates were recovered from 7 symptomatic Turkish olive cultivars on a semi -selective medium, PVF-1, and then identified as Psv by biochemical and molecular tests. The isolates produced characteristic gall symptoms on olive plants and were consistently re -isolated. Fatty acid methyl ester (FAME) analysis indicated that the major fatty acid components were oleic acid (18:1), palmitoleic acid (16:1), and palmitic acid (16:0), and also clustered the olive strains into 2 groups. The repetitive element palindromic PCR (rep-PCR) primer, the BOXA1R primer, produced the discriminatory profile, with amplicon sizes ranging from similar to 200 bp to 2 kb, and categorized the olive strains into 2 separate groups. Pulsed-Field Gel Electrophoresis (PFGE) differentiated the olive Psv isolates into 3 discrete haplotype groups after the genomic DNA was digested with Spa This is the first study using PFGE to determine the genetic diversity of the Psv olive population.