Efficient shoot regeneration and somatic embryogenesis from immature cotyledons of Brassica napus L.


Turgut K., BARGHCHI M., SCOTT R.

PLANT BREEDING, vol.117, no.5, pp.503-504, 1998 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 117 Issue: 5
  • Publication Date: 1998
  • Doi Number: 10.1111/j.1439-0523.1998.tb01984.x
  • Journal Name: PLANT BREEDING
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.503-504
  • Keywords: Brassica napus, immature cotyledon, shoot regeneration, somatic embryogenesis, EXPLANTS
  • Akdeniz University Affiliated: Yes

Abstract

Immature cotyledons of Brassica napus cv. 'Topas' were used to investigate the effects of various naphthaleneacetic acid and benzylaminopurine concentrations on morphogenesis in vitro. A high efficiency of adventitious shoot regeneration and somatic embryogenesis was achieved on Murashige and Skoog medium supplemented with the above growth regulators. Two types of somatic embryogenesis were obtained, the first directly and the second via a callus stage.