Akademik Veri Yönetim Sistemi
V. International Congress on Domestic Animal Breeding, Genetics and Husbandry - 2021 (ICABGEH-21), Lviv, Ukrayna, 27 - 30 Eylül 2021, ss.19
The purpose of this study was to look into the genetic polymorphism and association of milk protein genes and yield in Palestinian Awassi sheep strains. On a DNA level, the polymorphism of the beta lactoglobulin (β -LG) and Kappa casein (κ -CN) genes of two Awassi sheep reared in Palestine, known as indigenous (local) and improved Awassi sheep, was evaluated. The DNA of 54 sheep (23 native Awassi and 31 improved Awassi) was extracted and genotyped for ß-LG and κ -CN using by DNA sequencing. The amplified products for the ß-LG and κ -CN genes were 471 bp and 670 bp in size, respectively. Three genotypes were discovered in the ß-LG gene (AA, AB, and BB). In both local and improved Awassi sheep, the ß-LG sequence of exon 2 showed a point mutation (C>T) in the (X12817.1:c.112 T>C) locus. The existence of three different patterns, CC, TC, and TT, was discovered via κ -CN sequencing analysis. Furthermore, a single base pair mutation (C T) in exon 3 of codon 56 was discovered. Significant impacts of β -LG genotypes on fat content and density of milk were observed in both sheep, while a specific κ -CN genotype had effects on solid non-fat milk content exclusively in local Awassi. For both sheep, significant (P>0.05) differences in all measured parameters were observed between local and improved Awassi in the early season of milk, and between fat and lactose towards the end of the milk season. It is recommended to breed local Awassi sheep for outdoor rearing or extensive system in order to get more content of milk components.
Key words: Genetic polymorphism, β-lactoglobulin, κ-casein, DNA sequencing, Awassi