Çukurova Bölgesi Mısır Ekim Alanlarında Spıroplasma Ve Önemli Virüs Hastalık Etmenlerinin Belirlenmesi


Doç. Dr. HAKAN FİDAN

Tez Türü: Yüksek Lisans

Tezin Yürütüldüğü Kurum: Çukurova Üniversitesi, Fen Bilimleri Enstitüsü, Bitki Koruma, Türkiye

Tez Danışmanı: Prof. Dr. Mehmet Asil Yilmaz

Tezin Onay Tarihi: 2004

Desteklendiği Program: Diğer

Özet:

Streak, redding, yellowing, mosaic on the leaves, reduction on root growth and stunting were observed on the corn plants in corn growing area in 2001-2003 in Cukurova Region. At the end of three years survey in the corn plantation area severals samples were tested by DAS ELİSA against to several corn virus diseases and was concluded that Maize Dwarf Mosaic Virüsü (MDMV), Maize Mosaic Virüsü (MMV), Maize Stripe Virus (MSpV), and Barley Yellow Dvarf Virus (BYDV), are causal organism of corn plants in our Region. İnfection rate of MDMV, BYDV MSpV and MMV were determined %8,04 % 4,8, %1,8 and % 1,6 ile respectively. BYDV is first report on corn however  MSpV is first recorded plant virus diseases in Turkey.

cDNA was amplified by RT-PCR by using total RNA from MDMV infected corn plants. cDNA was multiplied with primers and the PCR products were run in electrophorisee The DNA band was found as a 670 bp on the gel.