Detection of Pathogenic Aeromonas hydrophila from Rainbow Trout (Oncorhynchus mykiss) Farms in Turkey

ÇAĞATAY İ. T., SEN E. B.

INTERNATIONAL JOURNAL OF AGRICULTURE AND BIOLOGY, cilt.16, sa.2, ss.435-438, 2014 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 16 Sayı: 2
  • Basım Tarihi: 2014
  • Dergi Adı: INTERNATIONAL JOURNAL OF AGRICULTURE AND BIOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.435-438
  • Anahtar Kelimeler: Aeromonas hydrophila, AHCYTOEN, Hly, OmpTS, VIRULENCE PROPERTIES, MULTIPLEX PCR, FISH, CLONING, EXPRESSION, SOBRIA, GENES, CHINA
  • Akdeniz Üniversitesi Adresli: Evet

Özet

The aim of this study was identifying and detecting virulent Aeromonas hydrophila, which caused bacterial hemorrhagic septicemia in rainbow trout (Oncorhynchus mykiss) farms in Mugla-Fethiye region by using PCR amplification of virulence-related genes. For this reason, clinically observed diseased fish samples were collected between 2009 and 2011. Three target genes, cytolytic enterotoxin (AHCYTOEN), hemolysin (Hly) and bacterial outer membrane protein (OmpTS) genes have been chosen for detecting virulent bacteria from those samples. Detected A. hydrophila from infected fish samples was demonstrated with positive amplifications of three genes at the same time. In addition, these positive results were separately confirmed by the amplification results of references strains. As a result, the infectious agent of this disease was identified rapidly by using the direct detection of bacteria through specific virulence determinants as genetic markers, before it spreads out. (C) 2014 Friends Science Publishers