A preliminary study of the dental implant therapy - Initial osteogenesis of human mesenchymal stem (HMS0014) cells on Titanium discs with different surface modifications

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Iwai Y., Matsuda Y., Nakatsuka M., Mikami Y., Kumabe S.

Okajimas Folia Anatomica Japonica, vol.88, no.4, pp.133-140, 2012 (Scopus) identifier identifier

  • Publication Type: Article / Article
  • Volume: 88 Issue: 4
  • Publication Date: 2012
  • Doi Number: 10.2535/ofaj.88.133
  • Journal Name: Okajimas Folia Anatomica Japonica
  • Journal Indexes: Scopus, BIOSIS, MEDLINE
  • Page Numbers: pp.133-140
  • Keywords: GBR, HMS0014 cell, In vitro, Osteogenesis, Peptide-based hydrogel scaffold
  • Akdeniz University Affiliated: Yes


HMS0014 cells were GBR-engineered to proliferate and differentiate into mature osteoblast(Ob)-like cells, which initiated hard tissue matrix deposition in both monolayer and PuraMatrix 3-D cultures. Subsequently, the osteogenesis initiated with attachment/adhesion of HMS0014 cells on either Titanium (Ti) or Ti alloy discs modified with osteoconductive/osteoinductive surface textures/substrates (e.g., Disc-AO, Disc-HA, Disc-SPI) was histologically assessed. The results obtained were as follows: 1) The HMS0014 cells actively proliferated and differentiated into mature Obs to initiate mineralisation of the ECM since day 1 in both monolayer and 3-D cultures; mineralization was prominently progressed between day 7 and day 14 of cultures. 2) The SEM of 60-minute(min)s specimens demonstrated a loose distribution of proliferating spherical-to-polygonal (10 to 40 μm in diameter, avg.) cells with a bulging cell body sending out many minute filopodia and some lamellipodia to attach with the substrate in the concavities. 3) In the 180-min specimens, the cultured HMS0014 cells actively proliferated and spread into flat, large polygonal cells with prominent lamellipodia and dendritic filopodia (30 μm × 90 μm to 100 μm × 200 μm, approx.) to employ cell-to-substrate and intercellular attachments. 4) On the other hand, the present immunohistochemistry of the attached HMS0014 cells demonstrated the co-expression of F-actin (actin filaments of the cytoskeleton) and CD51 (αV integrin) in both the 60-min and 180-min specimens. We concluded that the present GBR method enhanced HMS0014 cells to initiate an osteogenesis process with a direct bone-to-substratum contact on Ti discs which were subject to different surface modifications.