© 2022 by the authors.Determining how intermittently applied heat treatment during the preparation of vermicompost changes the effectiveness of the fertilizer is a challenge. In this study, organic Aloe vera was grown using heat-treated cattle manure vermicompost (IVC) and unheated cattle manure vermicompost (VC). Additionally, these two vermicomposts were combined with vermiwash (LV) and applied to the soil. Thus, the cumulative effect of vermicompost on soil biological properties (number of bacteria, dehydrogenase, urease, alkaline phosphatase, β-glycosidase) and plant growth (plant height, number of leaves, leaf biomass yield, number of suckers, fresh gel weight) was investigated. According to the results obtained, it was understood that HVC-30+LV, HVC-60+LV, and VC-60+LV applications were more effective on soil biological properties. On the other hand, HVC-30+LV and HVC-60+LV applications were found to be effective on plant growth. In addition, increases of 140% in soil bacterial number, 170% in dehydrogenase activity, 125% in urease activity, 122% in alkaline phosphatase activity, 123% in β-glycosidase activity, 65% in plant height, and 45% in leaf biomass yield and wet gel weight were observed. Accordingly, it can be stated that heat-treated cattle manure vermicompost applied to the soil at a rate of 30 t ha−1 together with vermiwash is beneficial for improving the biological properties of calcareous soil and for organic Aloe vera cultivation.