Immobilization stress in rat tissues: Alterations in protein oxidation, lipid peroxidation and antioxidant defense system


Sahin E., Gumuslu S.

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY, vol.144, no.4, pp.342-347, 2007 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 144 Issue: 4
  • Publication Date: 2007
  • Doi Number: 10.1016/j.cbpc.2006.10.009
  • Journal Name: COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.342-347
  • Keywords: immobilization stress, copper, zinc-superoxide dismutase, catalase, glutathione peroxidase, reduced glutathione, protein oxidation, lipid peroxidation, corticosterone, tissues, rat, ACID-REACTIVE SUBSTANCES, AGE-RELATED-CHANGES, GLUTATHIONE-PEROXIDASE, ENZYME-ACTIVITIES, SUPEROXIDE-DISMUTASE, RESTRAINT STRESS, NITRIC-OXIDE, COLD-STRESS, HEAT-STRESS, BRAIN
  • Akdeniz University Affiliated: Yes

Abstract

We determined the effects of immobilization stress on antioxidant status, protein oxidation and lipid peroxidation in brain, liver, kidney, heart and stomach of rats. Sixteen male Wistar rats (3 months old) were divided into controls (C) and immobilization stress group (IS). IS rats were immobilized for 190 min/day for 15 days. Plasma corticosterone levels were increased in IS group. Copper, zinc-superoxide dismutase activities were increased in brain, liver and kidney, but decreased in the heart and stomach after immobilization. Catalase activities were increased in brain, kidney and heart, and decreased in liver and stomach. Selenium-dependent glutathione peroxidase activities were decreased in brain and kidney, but increased in heart and stomach. Reduced glutathione levels were decreased, while protein carbonyl, conjugated dienes and thiobarbituric acid-reactive substances levels were increased in all tissues. Our results showed that the response of antioxidant defense system to stress differs for each tissue, and protein oxidation and lipid peroxidation is induced by immobilization stress in peripheral tissues. (c) 2006 Elsevier Inc. All rights reserved.