Comparison of genetic diversity of Turkish and Italian local chicken breeds for further conservation strategies

ÖZDEMİR D., MARETTO F., CASSANDRO M.

EUROPEAN POULTRY SCIENCE, cilt.80, 2016 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 80
  • Basım Tarihi: 2016
  • Doi Numarası: 10.1399/eps.2016.143
  • Dergi Adı: EUROPEAN POULTRY SCIENCE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Anahtar Kelimeler: Genetics, preservation, Turkish chicken, Italian chicken, microsatellite, population structure, genetic diversity, IN-SITU CONSERVATION, MICROSATELLITE MARKERS, POPULATION-STRUCTURE, NATIVE CHICKENS, PROGRAM, EVOLUTIONARY, VARIABILITY, SOFTWARE
  • Akdeniz Üniversitesi Adresli: Evet

Özet

The present study was conducted to estimate and compare the intra and inter genetic variability and population structure of 8 Mediterranean chicken populations based on two Turkish (Denizli and Gerze) and 6 Italian (Ermellinata di Rovigo, Padovana, Pepoi, Polverara, Robusta Lionata and Robusta Maculata) local chicken breeds, using 19 microsatellite markers. A total of 171 alleles were found across 19 microsatellite loci with a mean number of 9 alleles per locus. The breeds were highly differentiated with an average FST of 0.328. Global FIS values (0.277) indicated that non-random mating occurred in both Turkish and Italian chicken breeds. The mean number of alleles across breeds was 4.11. Observed heterozygosity was lower than HE for all breeds and it was in the range of 0.235 (Pepoi) to 0.421 (Ermellinata di Rovigo). Genetic distance (DC) between pairs of breeds ranged from 0.307 (Padovana and Polverara) to 0.716 (Gerze and Ermellinata di Rovigo), with average DC across breeds ranging from 0.451 (Padovana) to 0.687 (Gerze). The most probable structure clustering of the 8 chicken populations was at K = 10. While Turkish breeds showed no substructures within breeds and were observed to be distinct homogenous genetic populations, subgroups were observed mainly in Italian chicken breeds corresponding to the different ecotypes present in these breeds. The information resulting from this study may be used as an initial guideline to design further investigations for development of sustainable genetic improvement and conservation programs for Turkish and Italian chicken genetic resources.