A practical, low-cost, short-term storage method for genomic DNA

ÖZDEMİR D.

BIOTECHNIQUES, cilt.70, sa.4, ss.194-201, 2021 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 70 Sayı: 4
  • Basım Tarihi: 2021
  • Doi Numarası: 10.2144/btn-2020-0158
  • Dergi Adı: BIOTECHNIQUES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Aerospace Database, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Communication Abstracts, EMBASE, MEDLINE, Metadex, Veterinary Science Database, Directory of Open Access Journals, DIALNET, Civil Engineering Abstracts
  • Sayfa Sayıları: ss.194-201
  • Anahtar Kelimeler: cellulose, DNA preservation, dried blood spots, FTA, paper towel
  • Akdeniz Üniversitesi Adresli: Evet

Özet

The aim of this study was to assess the DNA preservation capability of cellulose paper towel and blotting paper as low-cost alternatives to commercial DNA preservation products. Chicken blood was applied as DNA source to each paper towel, blotting paper, FTA(R) cards and DNA/RNA Shield (TM). All samples were stored at room temperature for 130 days. DNA extraction from dried blood spots was performed after various time periods using Tris-EDTA and NaOH protocols. PCR activity and the mean amount of DNA isolated from paper towels were reliable. The results of this study demonstrated that cellulose-based blotting paper and especially paper towel had considerable DNA binding and preservation capacity for at least 130 days at room temperature without DNA degradation.