Serotyping Dichelobacter nodosus with PCR-SSCP


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Cagatay T. I., Hickford J.

JOURNAL OF ANIMAL AND VETERINARY ADVANCES, cilt.10, sa.13, ss.1678-1682, 2011 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 10 Sayı: 13
  • Basım Tarihi: 2011
  • Doi Numarası: 10.3923/javaa.2011.1678.1682
  • Dergi Adı: JOURNAL OF ANIMAL AND VETERINARY ADVANCES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1678-1682
  • Anahtar Kelimeler: Dichelobacter nodosus, fimA gene, PCR, Single Strand Conformational Polymorphism (SSCP), sequencing, sample, BACTEROIDES-NODOSUS, OVINE FOOTROT, BENIGN STRAINS, IDENTIFICATION, VIRULENT, SEQUENCE, SHEEP, ANTIGENS, VACCINE, DNA
  • Akdeniz Üniversitesi Adresli: Evet

Özet

In this study, we reported rapid approach for identification of D. nodosus serotypes that causes footrot, higly contagious diseases of the ruminants in worldwide with using Polymerase Chain Reaction-Single Strand Conformational Polymorphism (PCR-SSCP) analysis. A total of 500 swab samples from infected hoof lesion were investigated for presence of D. nodosus by PCR amplification of fimA gene. PCR-SSCP patterns revealed that each sample contained two or three different D. nodosus serotypes. Additionally on the of basis SSCP patterns, 8 different serogroups (A- G and I) were identified from New Zealand samples. These findings were confirmed by cloning and sequences of fimA gene.