Expressional Analyses of NM23-H1, KAI1 and MKK4 Metastasis-Related Genes in Metastatic Ovarian Carcinomas


BILGEN T., ERDOĞAN G., ŞİMŞEK T., GULKESEN H., Pestereli E., Karaveli S., ...More

TURKIYE KLINIKLERI TIP BILIMLERI DERGISI, vol.32, no.4, pp.984-989, 2012 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 32 Issue: 4
  • Publication Date: 2012
  • Doi Number: 10.5336/medsci.2011-26006
  • Journal Name: TURKIYE KLINIKLERI TIP BILIMLERI DERGISI
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.984-989
  • Keywords: Genital neoplasms, female, neoplasm metastasis, NM23-H1, KAI1, MKK4, omentum, TUMOR-SUPPRESSOR GENE, CANCER METASTASIS, SEROUS CARCINOMA, PROSTATE-CANCER, DOWN-REGULATION, PROTEIN, PROGRESSION, BREAST
  • Akdeniz University Affiliated: Yes

Abstract

Objective: Cytoreductive surgery as a basis of therapy in epithelial ovarian carcinomas (EOC) provides the primary tumor and metastatic tumor samples from a same patient. This gives an excellent opportunity for evaluation of metastatic factors by excluding inter-individual differences. Therefore, we aimed to define changes at mRNA levels of NM23-H1, KAI1 and MKK4 metastasis-related genes in the paired normal tissue, primary tumor and omental metastatic tumor samples obtained from a same patient. Material and Methods: mRNA levels were quantified by quantitative reverse transcription polymerase chain reaction (Q-RT-PCR) following total RNA extraction in normal tissues, primary malign tissues of EOC, and its metastatic lesions on omentum for 41 patients with stage III-C (FIGO) EOC. Results: We found that mRNA level of NM23-H1 was significantly higher in metastatic samples compared to primary tumors (p=0.009). On the other hand, MKK4 was found to be significantly lower in primary tumor samples compared to normal tissues (p=0.024). There was no significant change at mRNA level of KAI1 among normal tissues, primary tumors and omental metastatic tumor samples. Conclusion: We suppose that in detailed functional studies, approaches that suppress NM23-H1 gene and restore MKK4 gene would make these genes important molecular targets for treatment of metastatic EOC in the future.