IN VITRO ASSESSMENT OF DROUGHT TOLERANCE RESPONSES IN STRAWBERRY


ÜNAL N., TOZLU İ., NASIRCILAR A. G., ULUKAPI K.

FRESENIUS ENVIRONMENTAL BULLETIN, cilt.27, sa.12B, ss.9481-9486, 2018 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 27 Sayı: 12B
  • Basım Tarihi: 2018
  • Dergi Adı: FRESENIUS ENVIRONMENTAL BULLETIN
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED)
  • Sayfa Sayıları: ss.9481-9486
  • Anahtar Kelimeler: Fragaria x ananassa, in vitro, callus, SOD, CAT, MDA, callus dry weight, CHERRY ROOTSTOCK, WATER-STRESS, PLANTS
  • Akdeniz Üniversitesi Adresli: Evet

Özet

Global warming, intensifying problem in Mediterranean climate, has affect on yield and quality of crops. Strawberry is a sensitive crop to abiotic stresses (drought, salinity etc.) and this sensitivity is further increases in greenhouse production conditions. Hence, determinations to tolerance level to abiotic stress conditions of cultivars and genotypes are important for their cultivation and breeding. This tolerance level can be determined through morphological, physiological and biochemical analysis. In this study, tolerance levels of Osmanli and Festival strawberry cultivars have been determined through biochemical analysis (SOD- superoxide dismutase, CAT-catalase, MDA- malondialdehyde) of callus tissues and physiological analysis (callus formation ratio, callus fresh and dry weight, callus dry mass ratio) under in vitro drought conditions (0, 3, 6, 9 and 12% PEG). There was a linear relation between PEG concentration and oxidative enzyme activities (SOD and CAT) and lipid peroxidation (MDA). Moreover, there was no callus development at high PEG conditions (9-12% PEG 6000). The highest SOD, CAT and MDA values were obtained from Osmanli cultivar. In addition, the highest callus formation rate and fresh weight values were determined to Festival strawberry cultivar. Callus formation rates, callus fresh and dry weight decreased when PEG 6000 concentrations were increased on tried strawberry cultivars.