Mediterranean Agricultural Sciences, vol.35, no.3, pp.129-134, 2022 (Peer-Reviewed Journal)
The tomato plant is one of the most widely produced vegetables in the world. However, there
are several disease factors which limit tomato production. The Clavibacter michiganensis subsp.
michiganensis (Cmm) pathogen causes wilting and canker symptoms on the leaves of plants and
bird’s eye symptoms on the tomato and then the plants completely die. Development of resistant
tomato varieties is prerequisite due to absence of an effective control methods against the
bacterial disease. The resistant M3-9 and M3-15 tomato plants have been developed because of
mutation from susceptible NCEBR3 tomato seeds with ethyl methanosulfonate (EMS). For each
chromosome of the tomato genome, 24 SSR markers were selected from each end of the haploid,
12 tomato chromosomes, and polymorphic differences between susceptible and resistant tomato
plants were studied. Polymorphisms were found with SSR13 and SSRB18031 markers located
on chromosome 5 with resistant mutants, M3-9, M3-15 and susceptible original NCEBR3
plants. It is envisaged that a resistance gene is located on the 5th chromosome of resistant M3-
9 and M3-15 plants. Further fine mapping studies will reveal the location of the resistance
gene(s) for controlling bacterial canker and wilting pathogen.