Single Nucleotide Polymorphism and Production of IL-1 beta and IL-10 Cytokines in Febrile Seizures

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Nur B., Sahınturk D., Coşkun M., Duman Ö., Yavuzer U., Haspolat Ş.

NEUROPEDIATRICS, cilt.43, sa.4, ss.194-200, 2012 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 43 Sayı: 4
  • Basım Tarihi: 2012
  • Doi Numarası: 10.1055/s-0032-1323849
  • Dergi Adı: NEUROPEDIATRICS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.194-200
  • Anahtar Kelimeler: febrile seizure, cytokine, polymorphism, NECROSIS-FACTOR-ALPHA, GENE POLYMORPHISM, ANTIINFLAMMATORY CYTOKINES, INTERLEUKIN-1-BETA LEVELS, RECEPTOR ANTAGONIST, CEREBROSPINAL-FLUID, CONVULSIONS, CHILDREN, SUSCEPTIBILITY, ASSOCIATION
  • Akdeniz Üniversitesi Adresli: Evet

Özet

Inflammation and genetics may play a role in the pathogenesis of febrile seizures. The aim of this study was to investigate the spontaneous and lipopolysaccharide (LPS)induced production of IL-1 beta and IL-10, and the association between IL-1 beta (-511) and IL-10 (-1082) single nucleotide polymorphisms with LPS-induced cytokine production. The study included 92 febrile seizure patients and 132 healthy controls. First, we isolated genomic DNA and by using PCR-RFLP we genotyped the individuals for the cytokines gene polymorphism. Second, peripheral mononuclear cells of the individuals were isolated and stimulated with LPS to measure secretion capacity of IL-1 beta and IL-10 using specific ELISA kits. We found that both the IL-1 beta and IL-10 production was increased in febrile seizures. The rapid increase of IL-1 beta production in the supernatants of the LPS-induced cells was significantly higher at the fourth and the twenty-fourth hours in febrile and complex febrile seizures, respectively. The distribution of IL-10 (-1082) G allele differs significantly between cases and controls. The IL-1 beta (-511) G/A and the IL-10 (-1082) G/A genotype combination was found to be higher in patients with febrile seizure. Our results showed that IL-1 beta and IL-10 production was not influenced by the single nucleotide polymorphisms in the pathogenesis of febrile seizures.