ANALYSIS OF GENETIC DIVERSITY AMONG THE KIWIFRUIT (Actinidia spp.) GENOTYPES USING MOLECULAR MARKERS

İkten H., Şimşek C., Yirmibeş B., Bak T., Şenyurt M., Karadeniz T.

Bahçe, vol.49, pp.51-57, 2020 (Peer-Reviewed Journal)

  • Publication Type: Article / Article
  • Volume: 49
  • Publication Date: 2020
  • Journal Name: Bahçe
  • Journal Indexes: TR DİZİN (ULAKBİM)
  • Page Numbers: pp.51-57
  • Akdeniz University Affiliated: Yes

Abstract

To reveal genetic diversity and provide information to conserve valuable kiwifruit genotypes, 42 kiwifruit genotypes collected from Ordu province of Northern Turkey were analyzed using a set of 11 sequence-related amplified polymorphism (SRAP) and 7 inter-primer binding site (iPBS) retrotransposons primers. Out of 85 amplified bands, a total of 40 polymorphic bands were obtained with SRAP primers. iPBS primers produced a total of 71 bands 24 of which were polymorphic. An average 5 and 3.42 polymorphic bands were obtained for SRAP and iPBS markers respectively. The similarity coefficients were calculated, and cluster analysis was performed with (NTSYSpc 2.2). Dendrograms were constructed via UPGMA based on SRAP and iPBS markers. The dendrograms constructed using the SRAP and iPBS data exhibited two clusters. The Mantel test for comparison of the SRAP and iPBS-based similarity matrices showed moderate but significant correlation (r=0.55). Most of the genotypes showed a low range of genetic diversity and more than half of the genotypes found genetically closely related with similarity coefficient ranged from 0.90 to 1.00. The result of current study showed that there is need to increase the genetic diversity of kiwifruit using different breeding approach such as mutations, crossing and germplasm introduction. Keywords: Kiwifruit, genetic diversity, SRAP, iPBS, molecular characterization