Comparison of Four Methods to extract Bacterial DNA from Infected Fish Tissue

Tulay C. I.

RESEARCH JOURNAL OF BIOTECHNOLOGY, cilt.8, sa.10, ss.52-55, 2013 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 8 Sayı: 10
  • Basım Tarihi: 2013
  • Dergi Adı: RESEARCH JOURNAL OF BIOTECHNOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.52-55
  • Anahtar Kelimeler: FTA card, Renibacterium salmonarium, 16S rDNA, RENIBACTERIUM-SALMONINARUM, KIDNEY-DISEASE, PCR, SAMPLES, PAPER
  • Akdeniz Üniversitesi Adresli: Evet

Özet

This study is concerned with single-step bacterial DNA extraction of Renibacterium salmonarium from infected Rainbow Trout (Oncorhynchus mykiss) kidney tissue. We examined and compared four commonly used DNA extraction protocols to determine the reliable and practical method from tissue without isolating bacteria. A total of sixty extracted DNA samples from fish tissues have been tested to show their ability to amplification of R. salmonarium 16S rDNA gene region. Results of our comparison showed that the extraction of bacterial DNA from FTA card technology is more simple, reproducible and inexpensive.