Fertilization and embryo quality with and without artificial oocyte activation in teratozoospermia after intracytoplasmic morphologically selected sperm injection: a prospective randomized sibling-oocyte study

DİRİCAN E. K., Aksünger Ö., Erel L. R., Aydınuraz B.

ALPHA 2014, Scientists in Reproductive Medicine, 10th Biennial Conference, Antalya, Turkey, 9 - 11 May 2014, vol.28, no.1, pp.13-14

  • Publication Type: Conference Paper / Full Text
  • Volume: 28
  • City: Antalya
  • Country: Turkey
  • Page Numbers: pp.13-14
  • Akdeniz University Affiliated: Yes


The aim of this prospective randomized study using a sibling oocyte split design is to evaluate the effects of artificial oocyte activation (AOA) by calcium ionophore on the fertilization rates and embryo quality where intracytoplasmic morphologically selected sperm injection (IMSI) was used for sperm selection in teratozoospermia. Mature oocytes from 21 couples underwent assisted reproduction and intracytoplasmic sperm injection cycles in Memorial Antalya Hospital and diagnosed with severe teratozoospermia were enrolled in the present study. Mature oocytes were randomly allocated into two groups after IMSI. In the study group, half of the patients’ oocytes were exposed to AOA, and in the control group, AOA was not applied. Fertilization rates, the number of cleaved embryos and the number of grade A embryos on day 3, mean number of blastomeres and fragmentation rates were similar and not significant between study and control groups. In the AOA treated group, the number of cases with at least one grade A embryo was statistically significantly reduced compared to control group (61,9% vs. 95,24%; P=0,006, respectively). Our study reveals that AOA does not have any beneficial effect on fertilization rates after IMSI. Reduction of grade A embryos on day 3 may reflect a negative effect of AOA on in vitro embryo development. Use of AOA should be kept limited to cases with a known oocyte activating deficiency e.g. globozoospermia.