A new species, Cephalaria duzceensis N. Aksoy & R. S. Gokturk (Dipsacaceae) from western Black Sea region of Turkey is described. It is illustrated in line drawings and the morphology of the new species is compared with Cephalaria speciosa. Molecular and morphological methods were used to separate the two species. Both species were first identified morphologically and then studied by randomly amplified polymorphic DNA (RAPD) markers. Genetic similarities were calculated based on the RAPD data and used to construct a UPGMA dendrogram. According to the result, two main clusters were observed using the character differences. It is concluded that C duzceensis sp. nov. is different from C. speciosa.